Methacholine Challenge is a bronchial medical test used to assist in the diagnosis of asthma. The technician performing your exam will ask you to breathe in several doses of a methacholine solution, a drug which provokes bronchoconstriction, or narrowing of the airways. The degree of narrowing is calculated and the results used in diagnosing and treating any existing conditions the patient may have.
This test is ordered for patients who have normal pulmonary function tests, yet have symptoms or conditions such as:
- Persistent cough
- Wheezing
- Shortness of breath
- Asthma
Patient Instructions for Methacholine Challenge Testing:
Prior to your appointment date, we will give you detailed instructions and tell you what asthma medications to stop taking prior to the test. Antihistamines and inhalers interfere with the test results. Do not drink coffee for four hours prior to your test. Please allow 60 minutes for this test.

